Test Includes
Concentration of material and examination of specimen for ova and parasites by conventional iodine/saline and trichrome staining. This will not detect Cryptosporidium, Cyclospora cayetanensis, or Microsporidium.
Specimen Requirements
Specimen
Sputum or feces
Volume
3 to 4 mL sputum or 5 mL feces
Minimum Volume
3 mL (Note: This volume does not allow for repeat testing.)
Container
O & P transport container with formalin and PVA (Para-Pak® pink and gray)
Additional Information
Amebas and certain other parasites cannot be seen in stools containing barium. Amebic cysts, Giardia cysts, and helminth eggs are often recovered from formed stools. Mushy or liquid stools (either normally passed or obtained by purgation) often yield trophozoites. Purgation does not enhance the yield of Giardia. Formalin will preserve protozoan cysts and larvae and the eggs of helminths. It is used for concentration procedures. PVA will preserve the trophozoite stage of protozoa. A trichrome-stained smear may be prepared from PVA-fixed material. PVA cannot be concentrated; therefore, they should always be accompanied by a portion of the specimen in formalin.
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