Methodology
High-pressure liquid chromatography (HPLC) with tandem mass spectrometry
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Dihydrotestosterone and dihydrotestosterone/ratio may be used for differential diagnosis of diseases of sexual differentiation (DSD). DHT levels may be inappropriately low compared to testosterone levels in 5a-reductase deficiency. It is recommended by guidelines to measure the ratio of testosterone to DHT.
DHT levels may also be monitored to follow the effectiveness of 5a-reductase inhibitor treatment in female alopecia.
High-pressure liquid chromatography (HPLC) with tandem mass spectrometry
Test Details Use The primary clinical use of LH measurement is in evaluating the normalcy of hypothalamic-pituitary-gonadal axis. Measurement of serum gonadotropin levels will allow…
Test Details Use Levels of SHBG are under the positive control of estrogens and thyroid hormones, and are suppressed by androgens. These influences dynamically control…
Test Details Use Testosterone testing is used to evaluate androgen excess or deficiency related to gonadal function, adrenal function, or tumor activity. Testosterone levels may…
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